Work

New methods of structure refinement for macromolecular structure determination by NMR

Year: 1998

Type: article

Abstract: Recent advances in multidimensional NMR methodology have permitted solution structures of proteins in excess of 250 residues to be solved. In this paper, we discuss several methods of structure refinement that promise to increase the accuracy of macromolecular structures determined by NMR. These methods include the use of a conformational database potential and direct refinement against three-bond coupling constants, secondary 13C shifts, 1H shifts, T1/T2 ratios, and residual dipolar couplings. The latter two measurements provide long range restraints that are not accessible by other solution NMR parameters.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.95.11.5891

Authors: G. Marius Clore, Angela M. Gronenborn

Institutions: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases

Language: English

Topic: Advanced NMR Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2156995522

FWCI: 12.45

Cites: 63

Cited by: 233

Related to: 10