Year: 2000
Type: dissertation
Abstract: A new efficient expression system for Bacillus subtilis and its application to production of recombinant phytase.Submitted.Additional unpublised data are also presented.is described.Surprisingly, the enzyme differs from the well-characterized fungal phytases in many respects -sequence, enzyme characteristics and hydrolysis pathway.The enzyme has potential both in animal feed applications and in the production of specific myo-inositol phosphates.The data presented in this thesis together with very the recently published 3-D structure of Bacillus phytase (Ha et al., 2000) also implies a novel mode of phytic acid hydrolysis.An expression system for the production of the enzyme (as well as for other enzymes) in Bacillus is also presented in this thesis.This expression system proved to be very efficient, resulting in production levels exceeding most of those described in the literature.
Source: Työväentutkimus Vuosikirja
Author: Janne Kerovuo
Language: English
Topic: Phytase and its Applications
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2157014544