Work

Culturing Human Embryonic Stem Cells in Feeder-Free Conditions: Figure 1.

Year: 2008

Type: preprint

Abstract: INTRODUCTIONHuman embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs without feeder cell contamination.

Source: Cold Spring Harbor Protocols

DOI: 10.1101/pdb.prot5044

Authors: Sohyun L. McElroy, Renee A. Reijo Pera

Institutions: Institute for Stem Cell Biology and Regenerative Medicine, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2157149580

Cites: 3

Cited by: 14

Related to: 10