Year: 2003
Type: article
Abstract: We have generated an optimized inducible recombination system for conditional gene targeting based on a Cre recombinase-steroid receptor fusion. This configuration allows efficient Cre-mediated recombination in most organs of the mouse upon induction, without detectable background activity. An ES cell line, was established that carries the inducible recombinase and a loxP-flanked lacZ reporter gene. Out of this line, completely ES cell-derived mice were efficiently produced through tetraploid blastocyst complementation, without the requirement of mouse breeding. Our findings provide a new concept allowing the generation of inducible mouse mutants within 6 months, as compared to 14 months using the current protocol.
Source: Nucleic Acids Research
DOI: 10.1093/nar/gng012
Authors: J. Seibler, Branko Zevnik, Birgit Küter-Luks, Susanne Andreas, Heidrun Kern, Thomas Hennek, Anja Rode, Cornelia Heimann, Nicole Faust, Gunther Kauselmann, Michael Schoor, Rudolf Jaenisch, Klaus Rajewsky, Ralf Kühn, Frieder Schwenk
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2157182132