Work

Development of a Singleplex PCR Assay for Rapid Identification and Differentiation of Cryptococcus neoformans var.grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and Hybrids

Year: 2013

Type: article

Abstract: A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.00064-13

Authors: Xiaobo Feng, Xiaohua Fu, Bo Ling, Lei Wang, Wanqing Liao, Zhirong Yao

Institutions: Shanghai Jiao Tong University, XinHua Hospital, Tongji University, Second Military Medical University, Shanghai Changzheng Hospital

Language: English

Topic: Fungal Infections and Studies

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2157319636

FWCI: 3.893

Cites: 20

Cited by: 38

Related to: 10