Myogenic MicroRNA Expression Requires ATP-Dependent Chromatin Remodeling Enzyme Function
Year: 2010
Type: article
Abstract: Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.
Source: Molecular and Cellular Biology
DOI: 10.1128/mcb.00214-10
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: Ministry of Education, Culture, Sports, Science and Technology, National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke
Awards: G1190947309, G2677314950, Transcription Factor Function in Chromatin, Control of Papillomavirus Expression and Transformation, G4543602410, Diabetes Endocrinology Research Center (DERC), Molecular Analysis of Hindbrain Formation, G9000110905, G9000193114, Molecular Analysis of Hindbrain Development
OpenAlex ID: W2157647985