The Role of Bone Marrow Mononuclear Cells in Angiogenesis in Mouse Hind Limb Ischemic Model
Year: 2007
Type: article
Abstract: Background: Angiogenesis is enhanced in the ischemic tissues after the injection of bone marrow cells (BMCs).However the exact mechanisms for this are not yet fully understood.Methods: A unilateral ischemic limb was surgically induced in mice and then BMCs were injected into the ischemic area.We measured the capillary/muscle ratio.Fluorescence-labeled BMCs were injected into the ischemic tissues and then the locations of the cells were examined by using a confocal microscope.Recruitment of bone marrow-derived cells into the ischemic tissue was examined in a sex-mismatched bone marrow transplantation (BMT) setting by identifying the Y chromosome with using the FISH technique.The expressions of VEGF, MMP-9, SDF-1 and CXCR-4 were measured by Western blot analysis.Results: The capillary/muscle ratio was more increased in the BMC-injected group than in the control group (P＜0.05).Florescence-labeled BMCs, which had been directly injected into ischemic tissue, were not detected in the tissue.In the sex-mismatched bone marrow transplantation models, the ischemic tissues of the BMC-injected group recruited a much greater number of Y chromosome-positive bone marrowderived cells, as compared to the control group.The expressions of VEGF and MMP-9 were increased after injection of BMCs.SDF-1 was expressed on the seventh day in the BMC-injected group and CXCR-4 was highly expressed until 12 weeks in the BMC-injected group.Conclusion: We suggest that the injection of BMCs into ischemic tissue recruits CXCR-4-positvie cells from the bone marrow via the up-regulation of VEGF, MMP-9 and SDF-1, and these CXCR-4-positive cells may play a role in neovascularization.(
Source: The Korean Journal of Hematology
Institution: Catholic University of Korea
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W2157655246