Work

In Vitro and in Vivo Formation of Two New Metabolites of Melatonin

Year: 1974

Type: article

Abstract: A partially purified enzyme from rabbit brain catalyzed the oxygenative cleavage of the pyrrole moiety of melatonin yielding Nr-acetyl-NZ-formyl-5-methoxykynurenamine, which was further degraded to NY-acetyl-5-methoxykynurenamine by the action of formamidase.Authentic samples of these two metabolites were synthesized by ozonolysis of melatonin and were shown to be indistinguishable from the enzymic products.When [14C]melatonin was injected intracisternally, the major metabolite in the rat brain was shown to be NY-acetyl-S-methoxykynurenamine.The results indicate that these two new metabolites are involved in the major metabolic pathway of melatonin in the central nervous system.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)42976-1

Authors: Fusao Hirata, Osamu Hayaishi, Takashi Tokuyama, Siro Senoh

Institutions: Kyoto University, Osaka City University, Central Research Institute, Suntory (Japan)

Language: English

Topic: Circadian rhythm and melatonin

Subfield: Endocrine and Autonomic Systems

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W2157835171

FWCI: 6.904

Cites: 9

Cited by: 323

Related to: 10