Year: 2010
Type: article
Abstract: expensive to buy or maintain for a single-researcher laboratory, with the exception of the Polonator G. and the recently announced Junior, a next-generation bench-top pyrosequencer to be marketed in for small to medium labs.Sequencing facilities will perform much of the benchwork of sample preparation, which can be tricky depending on the platform, as part of the sequencing service for a modest fee.To choose the most appropriate platform for a project, some knowledge of the differences among methods is useful.Here, we briefly describe the methods and the unique benefits and drawbacks of each platform (see Table ).There are several reviews that describe the technologies in greater detail, as well as animated demonstrations on the company websites (Hudson , Mardis , Shendure and Ji ; see Table for company websites).Commercial next-generation sequencing methods can be distinguished by the role of PCR in library preparation.Four main platforms are amplification-based: Roche GS FLX, Illumina Genome Analyzer IIx, ABI SOLiD Plus System, and Polonator G. (Table ).Two single-molecule sequencing methods (i.e., not PCR-based) are either very recently available or nearly available: Helicos Genetic Analysis System and Pacific Biosciences SMRT technology, respectively (Table ).
Source: The Auk
Authors: Heather Lerner, Robert Fleischer
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Funder: National Science Foundation
OpenAlex ID: W2157917286