Work

Splicing enhances translation in mammalian cells: an additional function of the exon junction complex

Year: 2004

Type: preprint

Abstract: In mammalian cells, spliced mRNAs yield greater quantities of protein per mRNA molecule than do otherwise identical mRNAs not made by splicing. This increased translational yield correlates with enhanced cytoplasmic polysome association of spliced mRNAs, and is attributable to deposition of exon junction complexes (EJCs). Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA. Thus, in addition to its previously characterized role in NMD, the EJC also promotes mRNA polysome association. Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD.

Source: Genes & Development

DOI: 10.1101/gad.1163204

Authors: Ajit Nott, Hervé Le Hir, Melissa J. Moore

Institutions: Howard Hughes Medical Institute, Brandeis University

Language: English

Topic: RNA Research and Splicing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences

Award: Pre-mRNA Splicing Mechanisms