Year: 2003
Type: article
Abstract: Mass spectrometry is now widely used in many areas of biomolecule analysis and this diversity is well represented by the analysis of oligonucleotides, carbohydrates and steroids. In this paper an overview of the common techniques used for analysis of these biomolecules is presented, including electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI), desorption/ionization on silicon (DIOS), and negative chemical ionization gas chromatography mass spectrometry (NCI GC/MS). Additionally, important aspects of biomolecule analysis, such as molecular weight determination, sequencing using tandem mass spectrometry and enzymes, precursor ion scanning, noncovalent interactions, and sensitivity, will be discussed.
Source: Journal of Spectroscopy
DOI: 10.1155/2003/725480
Authors: Bruce Jon Compton, Gary Siuzdak
Institutions: Waters (United States), Scripps Research Institute
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $1,630
OpenAlex ID: W2158235630