Transformation of trehalose synthase gene (TPS Gene) into corn inbred line and identification of drought tolerance
Year: 2011
Type: article
Abstract: Trehalose synthase gene (TPS gene) was transferred into maize inbred line Zheng58 with the improved pollen tube path method. At the three to five leaf stage, selection with spray Basta (0.7%) solution was conducted, and was then followed by polymerase chain reaction (PCR) and PCR-southern detection. The results showed that the new gene was transferred into the plants and integrated into the plant chromosomes. The initial drought-tolerance test in farmland confirmed that the drought resistance of transgenic plants was higher than that of non-transgenic plants. The results show that the new maize drought resistance germplasm can be gained by means of pollen tube path which is a feasible, effective and new way for maize drought resistance breeding. Key words: Maize, trehalose synthase gene (TPS Gene), drought resistance.
Source: AFRICAN JOURNAL OF BIOTECHNOLOGY
DOI: 10.5897/ajb.9000502
Authors: Chunlin Dong, Mingyi Zhang, Zhang Yan-qin, Lili Yang, Liang Gaimei, Jie Sun, Zhongping Lin, Gou Jjian-fang
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2158241326