Work

Novel Methyltransferase for Modified Uridine Residues at the Wobble Position of tRNA

Year: 2003

Type: article

Abstract: We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.23.24.9283-9292.2003

Authors: Hamid R. Kalhor, Steven Clarke

Institution: University of California, Los Angeles

Language: English

Topic: RNA modifications and cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences

Awards: G2558415806, CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION, CONTROL OF EUCARYOTIC FUNCTION BY METHYLATION, ENYZMES AFFECTING THE ACCUMULATION OF ALTERED PROTEINS, G9000195295