Work

Reactivation of inactive X chromosome and post-transcriptional reprogramming of Xist in induced pluripotent stem cells

Year: 2014

Type: article

Abstract: Direct reprogramming of somatic cells to pluripotent stem cells entails the obliteration of somatic cell memory and the reestablishment of epigenetic events. Induced pluripotent stem cells (iPSCs) have been created by reprogramming somatic cells through the transduction of reprogramming factors. During cell reprogramming, female somatic cells must overcome at least one more barrier than male somatic cells in order to enter a pluripotent state, as they must reactivate an inactive X chromosome (Xi). In this study, we investigated whether the sex of somatic cells affects reprogramming efficiency, differentiation potential and the post-transcriptional processing of Xist RNA after reprogramming. There were no differences between male and female iPSCs with respect to reprogramming efficiency or their differentiation potential in vivo. However, reactivating Xi took longer than reactivating pluripotency-related genes. We also found that direct reprogramming leads to gender-appropriate post-transcriptional reprogramming - like male embryonic stem cells (ESCs), male iPSCs expressed only the long Xist isoform, whereas female iPSCs, like female ESCs, expressed both the long and short isoforms.

Source: Journal of Cell Science

DOI: 10.1242/jcs.154294

Authors: Jong Soo Kim, Hyun Woo Choi, Marcos J. Araúzo-Bravo, Hans R. Schöler, Jeong Tae Do

Institutions: Konkuk University, Ikerbasque, Biogipuzkoa Health Research Institute, Max Planck Institute for Molecular Biomedicine

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Rural Development Administration, National Research Foundation of Korea

OpenAlex ID: W2159504778