Year: 2012
Type: article
Abstract: Viral hemorrhagic septicemia virus (VHSv), a member of the family Rhabdoviridae, is a highly contagious fish virus responsible for large-scale fish die-offs worldwide.A new strain of VHSv, designated IVb, has recently spread to the Great Lakes threatening the tourism, sports fishing and fishery industries of the region.Research on virus-host interactions in VHSv infected fish has been mostly limited to population and ecological based studies while the molecular basis of the disease remains widely uncharacterized.To study virus-host interactions on a molecular level, we cloned four of the six VHSv genes including the matrix (m), nucleocaspid (n), non-virion (nv), and phosphoprotein (p) genes.Of primary interest, the M protein encoded by VHSv seems to share similar characteristics with the matrix protein of a related rhabdovirus.Comparable to the wellstudied rhabdovirus vesicular stomatitis virus (VSV) M protein, ectopically expressed VHSv M inhibits promoter activity of both an interferon stimulated response element containing promoter and a constitutively active simian vacuolating virus 40 (SV40) promoter in Epithelioma Papulosum Cyprini (EPC) cells.Interestingly, real-time PCR
Author: Adam Pore
Institution: University of Toledo
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2159716022