Work

Differential amplification of rRNA genes by polymerase chain reaction

Year: 1992

Type: article

Abstract: The polymerase chain reaction (PCR) is used widely to recover rRNA genes from naturally occurring communities for analysis of population constituents. We have found that this method can result in differential amplification of different rRNA genes. In particular, rDNAs of extremely thermophilic archaebacteria often cannot be amplified by the usual PCR methods. The addition of 5% (wt/vol) acetamide to a PCR mixture containing both archaebacterial and yeast DNA templates minimized nonspecific annealing of the primers and prevented preferential amplification of the yeast small-subunit rRNA genes.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.58.10.3417-3418.1992

Authors: A L Reysenbach, L J Giver, G S Wickham, N R Pace

Institutions: Indiana University Bloomington, Indiana University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G5314457195