Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum.
Year: 1982
Type: article
Abstract: A rapid and simple method for the isolation of membranes from subcellular organelles is described. The procedure consists of diluting the organelles in ice-cold 100 mM Na2CO3 followed by centrifugation to pellet the membranes. Closed vesicles are converted to open membrane sheets, and content proteins and peripheral membrane proteins are released in soluble form. Here we document the method by applying it to various subfractions of a rat liver microsomal fraction, prepared by continuous density gradient centrifugation according to Beaufay et al. (1974, J. Cell Biol. 61:213-231). The results confirm and extend those of previous investigators on the distribution of enzymes and proteins among the membranes of the smooth and rough endoplasmic reticulum. In the accompanying paper (1982, J. Cell Biol. 93:103-110) the procedure is applied to peroxisomes and mitochondria.
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.93.1.97
Authors: Y Fujiki, A L Hubbard, S Fowler, P B Lazarow
Institutions: Johns Hopkins University, Rockefeller University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2160107586