Artificial Induction of Oocyte Maturation and Development in the Sea Cucumbersholothuria Leucospilotaandholothuria Pardalis
Year: 1980
Type: article
Abstract: Isolated oocytes of the sea cucumbers Holothuria leucospilota and Holothuria pardalis were induced to mature with 2,3-dimercapto-1-propanol (BAL), dithiothreitol (DTT) and L-cysteine. 1-Methyladenine, known as the maturation-inducing hormone of starfishes, failed to induce oocyte maturation of the sea cucumbers. Observation of the maturation process revealed that the germinal-vesicle of the oocyte treated with DTT migrated to the micropyle process and broke down there, where polar bodies were subsequently formed. After insemination, mature eggs obtained with DTT treatment developed to the typical auricularia larvae.
Source: Biological Bulletin
DOI: 10.2307/1540860
Author: YOSHIHIKO K. MARUYAMA
Institution: Kyoto University
Language: English
Subfield: Aquatic Science
Domain: Life Sciences
SDG: Life below water
Open Access status: green
OpenAlex ID: W2160858330