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In Vivo Targeting of Hydrogen Peroxide by Activatable Cell-Penetrating Peptides

Year: 2013
Type: article
Abstract: A hydrogen peroxide (H2O2)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H2O2 through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H2O2 sensor selectively reacts with endogenous H2O2 in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress.
DOI: 10.1021/ja411547j
Language: English
Subfield: Spectroscopy
Field: Chemistry
Open Access status: hybrid
Estimated APC paid: $4,500
Funders: U.S. Department of Defense, Howard Hughes Medical Institute, American Asthma Foundation, National Institutes of Health, National Heart, Lung, and Blood Institute, National Cancer Institute, National Institute of General Medical Sciences
Awards: G4119534169, Injectable reporters to image tumors and guide resection, UCSD Cancer Nanotechnology Training Center, G5850682332, HYBRID NANOTECHNOLOGIES FOR DETECTION AND SYNERGISTIC THERAPIES FOR BREAST CANCER, Exploiting Protease-Activatable Agents to Characterize and Treat Asthma, Medical Scientist Training Program
OpenAlex ID: W2161146618
FWCI: 8.071
Cites: 43
Cited by: 243
Related to: 10