Work

Amplification of the Gene for Histidyl-tRNA Synthetase in Histidinol-Resistant Chinese Hamster Ovary Cells

Year: 1985

Type: article

Abstract: Histidinol-resistant (HisOHR) mutants with up to a 30-fold increase in histidyl-tRNA synthetase activity have been isolated by stepwise adaptation of wild-type Chinese hamster ovary (CHO) cells to increasing amounts of histidinol in the medium. Immunoprecipitation of [35S]methionine-labeled cell lysates with antibodies to histidyl-tRNA synthetase showed increased synthesis of the enzyme in histidinol-resistant cells. The histidinol-resistant cell lines had an increase in translatable polyadenylated mRNA for histidyl-tRNA synthetase. A cDNA for CHO histidyl-tRNA synthetase has been cloned, using these histidyl-tRNA synthetase-overproducing mutants as the source of mRNA. Southern blot analysis of wild-type and histidinol-resistant cells with this cDNA showed that the histidyl-tRNA synthetase DNA bands were amplified in the resistant cells. These HisOHR cells owed their resistance to histidinol to amplification of the gene for histidyl-tRNA synthetase.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.5.9.2381

Authors: F W Tsui, I L Andrulis, H Murialdo, L Siminovitch

Institutions: University of Toronto, Mount Sinai Hospital, Mount Sinai Hospital, Hospital for Sick Children, Mount Sinai Hospital

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, Medical Research Council, National Cancer Institute

OpenAlex ID: W2161573420

FWCI: 1.179

Cites: 26

Cited by: 23

Related to: 10