Year: 1986
Type: review
Abstract: They sought it with thimbles, they sought it with care; They pursued it with forks and hopes (6) A Definition and History Not so long ago, in the olden (golden?) days, scientists interested in the biochemistry and cell biology of proteins attempted to explain an enzyme's catalytic and regulatory functions and the cytoskeleton's properties in terms of protein structure. But SDS PAGE, monoclonal antibodies, and cDNA libraries (nucleic acid sequences reverse transcribed from mRNAs and inserted into the genomes of bacteriophage or plasmids) changed things, and many of us now study proteins the other way around. We know structure, in the form of subunit molecular weights, isoelectric points, modifications by phosphate, sugars, methylations, etc., and in some cases, even primary sequences and the precise localization in the
Source: The Journal of Cell Biology
Author: M J Schlesinger
Institution: Washington University in St. Louis
Language: English
Topic: Heat shock proteins research
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2161604361