Work

Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa

Year: 2005

Type: article

Abstract: A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.71.3.1642-1647.2005

Authors: Ryan Woodyer, Michael Simurdiak, Wilfred A. van der Donk, Huimin Zhao

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Enzyme Production and Characterization

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green