Work

Localization of Cap-Binding Protein in Subcellular Fractions of HeLa Cells

Year: 1982

Type: article

Abstract: The 26,000-M(r) cap-binding protein was analyzed by a cross-linking assay in cell fractions from uninfected and poliovirus-infected HeLa cells. Cap-binding protein was found in the postribosomal supernatant (S-200) and in the ribosomal salt wash. The cap-binding protein in the S-200 had a sedimentation coefficient of 5 to 7S and lacked the ability to restore translation in extracts of poliovirus-infected cells.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.2.12.1639

Authors: Joanna L. Hansen, Diane O. Etchison, John W. B. Hershey, Ellie Ehrenfeld

Institutions: University of Utah, University of California, Davis

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: National Institutes of Health