Work

DNA sequences required for regulated expression of β-globin genes in murine erythroleukaemia cells

Year: 1984

Type: article

Abstract: We have introduced into murine erythroleukaemia (MEL) cells a series of human globin gene cosmids and two sets of hybrid genes constructed from the human beta-globin gene and the human gamma-globin or murine H-2Kbm1 genes. S1-nuclease analysis of the mRNA products from these genes before and after MEL cell differentiation showed that the human beta-globin gene, but not the human epsilon- or gamma-globin or H-2Kbm1 genes, is induced specifically. Hybrid genes containing human beta-globin DNA sequences from either 5' or 3' side of the translation initiation site were both inducible. Measurement of the relative rate of transcription showed this induction to be the result of transcriptional activation. We therefore suggest that DNA sequences which regulate beta-globin gene expression during MEL differentiation are located both 5' and 3' to the translation initiation site.

Source: Philosophical transactions of the Royal Society of London. Series B, Biological sciences

DOI: 10.1098/rstb.1984.0127

Authors: Sewall Wright, E. deBoer, A. Rosenthal, Richard Anthony Flavell, Franklin Gerardus Grosveld

Institutions: Hebrew University of Jerusalem, Hadassah Medical Center, Biogen (United States)

Language: English

Topic: Hemoglobinopathies and Related Disorders

Subfield: Genetics

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Hebrew University of Jerusalem, Medical Research Council

OpenAlex ID: W2162964975

FWCI: 2.44

Cites: 27

Cited by: 31

Related to: 10