Work

Confocal optical microscopy

Year: 1996

Type: article

Abstract: Confocal optical microscopy is a technique for increasing the contrast of microscope images, particularly in thick specimens. By restricting the observed volume, the technique keeps overlying or nearby scatterers from contributing to the detected signal. The price for this is that the instrument must observe only one point at a time (in the scanning laser version) or a group of separated points with very little light (in the disc version). This paper describes how the confocal advantage comes about and how it is implemented in actual instruments. This review was received in October 1995

Source: Reports on Progress in Physics

DOI: 10.1088/0034-4885/59/3/003

Author: Robert H Webb

Institution: Massachusetts General Hospital

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: U.S. Department of Energy, University of Cambridge

Award: G222768804