Work

Carbon catabolite repression in Thermoanaerobacterium saccharolyticum

Year: 2012

Type: article

Abstract: BACKGROUND: The thermophilic anaerobe Thermoanaerobacterium saccharolyticum is capable of directly fermenting xylan and the biomass-derived sugars glucose, cellobiose, xylose, mannose, galactose and arabinose. It has been metabolically engineered and developed as a biocatalyst for the production of ethanol. RESULTS: We report the initial characterization of the carbon catabolite repression system in this organism. We find that sugar metabolism in T. saccharolyticum is regulated by histidine-containing protein HPr. We describe a mutation in HPr, His15Asp, that leads to derepression of less-favored carbon source utilization. CONCLUSION: Co-utilization of sugars can be achieved by mutation of HPr in T. saccharolyticum. Further manipulation of CCR in this organism will be instrumental in achieving complete and rapid conversion of all available sugars to ethanol.

Source: Biotechnology for Biofuels

DOI: 10.1186/1754-6834-5-85

Authors: Vasiliki Tsakraklides, A Joe Shaw, Bethany B Miller, David A Hogsett, Christopher D Herring

Institution: Masco (United States)

Language: English

Topic: Microbial Metabolic Engineering and Bioproduction

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,990

Funder: U.S. Department of Energy