Work

Localization of Bud2p, a GTPase-activating protein necessary for programming cell polarity in yeast to the presumptive bud site

Year: 1999

Type: preprint

Abstract: Yeast cells of different cell type exhibit distinct budding patterns that reflect the organization of the actin cytoskeleton. Bud1p (Rsr1p), a Ras-like GTPase, and Bud2p, a GTPase-activating protein for Bud1p, are essential for proper budding pattern. We show that Bud2p is localized at the presumptive bud site in G(1) cells in all cell types and that this localization is independent of Bud1p. Bud2p subsequently localizes to the mother-bud neck after bud emergence; this localization depends on the integrity of the septins. These observations indicate that Bud2p becomes positioned in G(1) cells by recognizing cell type-specific landmarks at the presumptive bud site.

Source: Genes & Development

DOI: 10.1101/gad.13.15.1912

Authors: H.-O. Park, A. Sanson, I. Herskowitz

Institutions: University of California, San Francisco, The Ohio State University

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: American Cancer Society, March of Dimes Foundation, National Institutes of Health, National Institute of General Medical Sciences

Awards: GENETIC CONTROL OF CELL POLARITY IN BUDDING YEAST, G340362463, SPATIAL CONTROL OF CELL POLARITY DURING YEAST BUDDING