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Identification of Staphylococcus Species Directly from Positive Blood Culture Broth by Use of Molecular and Conventional Methods

Year: 2009

Type: article

Abstract: We compared two real-time PCR assays (both by the use of melting curve analysis) for their ability to identify Staphylococcus species directly from 200 positive blood culture bottles. The PCR assays correctly identified 83% to 94% of the Staphylococcus isolates to species clusters. Molecular testing significantly outperformed commercially available latex tests (sensitivity for both latex tests, <15%) when it was used directly with broth from signal-positive blood cultures.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.01850-08

Authors: Maitry S. Mehta, Suzanne M. Paule, Richard B. Thomson, Karen L. Kaul, Lance R. Peterson

Institutions: Endeavor Health, Northwestern University

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2164118608

FWCI: 1.278

Cites: 23

Cited by: 11

Related to: 10