Regulation of the Rhp26 ERCC6/CSB chromatin remodeler by a novel conserved leucine latch motif
Year: 2014
Type: article
Abstract: CSB/ERCC6 (Cockayne syndrome B protein/excision repair cross-complementation group 6), a member of a subfamily of SWI2/SNF2 (SWItch/sucrose nonfermentable)-related chromatin remodelers, plays crucial roles in gene expression and the maintenance of genome integrity. Here, we report the mechanism of the autoregulation of Rhp26, which is the homolog of CSB/ERCC6 in Schizosaccharomyces pombe. We identified a novel conserved protein motif, termed the "leucine latch," at the N terminus of Rhp26. The leucine latch motif mediates the autoinhibition of the ATPase and chromatin-remodeling activities of Rhp26 via its interaction with the core ATPase domain. Moreover, we found that the C terminus of the protein counteracts this autoinhibition and that both the N- and C-terminal regions of Rhp26 are needed for its proper function in DNA repair in vivo. The presence of the leucine latch motif in organisms ranging from yeast to humans suggests a conserved mechanism for the autoregulation of CSB/ERCC6 despite the otherwise highly divergent nature of the N- and C-terminal regions.
Authors: Lanfeng Wang, Oliver Limbo, Jia Fei, Lu Chen, Bong Kim, Jie Luo, Jenny Chong, Ronald C. Conaway, Joan W. Conaway, Jeff A. Ranish, James T. Kadonaga, Paul Russell, Dong Wang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: Structural Basis of Transcriptional Mutagenesis and Arrest of RNA Polymerase II, Chromatin Dynamics and Gene Expression, G1409347209, G1675169588, Biochemistry of Eukaryotic Messenger RNA Synthesis, DETECTION AND MODELS OF TOXICANT EXPOSURE, G3617295572, G4291047071, G4970819608, Mapping the Dynamic Architecture of the Human Mediator Complex, G5147361085, BIOCHEMISTRY OF EUKARYOTIC MESSENGER RNA SYNTHESIS, Double-Strand Break Repair and Genome Stability, Structural Basis of Transcriptional Mutagenesis and Arrest of RNA Polymerase II, Mre11/Rad50/Nbs1 Structural Biology for DNA Damage Responses, G6653922034, G6846307619, Checkpoint Responses for Protecting Genome Integrity in S-Phase, Quantitative Systems Biology, Molecular Mechanisms for DNA Damage Processing by Transcription Machinery
OpenAlex ID: W2164360591