Work

Analysis and Optimization of Copper‐Catalyzed Azide–Alkyne Cycloaddition for Bioconjugation

Year: 2009

Type: article

Abstract: How to click with biomolecules: Copper-catalyzed azide–alkyne cycloaddition has been optimized for use with biological molecules. The key development is the addition of two reagents that allow ascorbate to be used as reducing agent whilst eliminating problems caused by copper ascorbate side reactions. The result is a robust, rapid, and convenient procedure for the modification of proteins, DNA, RNA, and other biomolecules (see scheme).

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200905087

Authors: Vu Hong, Stanislav I. Presolski, Celia Ma, M. G. Finn

Institution: Scripps Research Institute

Language: English

Topic: Click Chemistry and Applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Center for Research Resources

Awards: G7306699446, G8101635193