Enantioselective Intramolecular CH Amination Catalyzed by Engineered Cytochrome P450 Enzymes In Vitro and In Vivo
Year: 2013
Type: article
Abstract: Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into CH bonds, their ability to use nitrogen for the same purpose has so far not been explored. Engineered variants of cytochrome P450BM3 have now been found to catalyze intramolecular CH aminations in azide substrates. Mutations to two highly conserved residues significantly increased this activity.
Authors: John A. McIntosh, Pedro S. Coelho, Christopher C. Farwell, Z. Jane Wang, Jared C. Lewis, Tristan R. Brown, Frances H. Arnold
Institutions: California Institute of Technology, University of Chicago
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2165365513