Detection of Fastidious Vaginal Bacteria in Women with HIV Infection and Bacterial Vaginosis
Year: 2009
Type: article
Study design: Observational Study
Abstract: BACKGROUND: Fastidious bacteria have been associated with bacterial vaginosis (BV) using PCR methods. We assessed the prevalence of these bacteria in HIV-1 infected women and their relationship with vaginal pH and shedding of HIV-1 RNA. METHODS: 64 cervicovaginal lavage (CVL) samples were collected from 51 women. Vaginal microbiota were characterized using 8 bacterium-specific quantitative PCR assays. RESULTS: Women with the fastidious bacteria Bacterial Vaginosis Associated Bacterium (BVAB) 1, 2, and 3 showed a trend to increased HIV-1 shedding (OR 2.59-3.07, P = .14-.17). Absence of Lactobacillus crispatus (P < .005) and presence of BVAB2 (P < .001) were associated with elevated vaginal pH. BVAB1, 2, and 3 were highly specific indicators of BV in HIV-infected women, with specificities of 89%-93%. CONCLUSIONS: Fastidious bacteria (BVAB 1, 2, and 3) remain specific indicators of BV in HIV-infected women, and BVAB2 may contribute to the elevated vaginal pH that is a hallmark of this syndrome.
DOI: 10.1155/2009/236919
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $820
Awards: G1230579671, G1524021829, The Role of Uncultivated Bacteria in Bacterial Vaginosis, G2197042240, Antiviral Therapy and HIV in the Genital Tract of Women, Antiviral Therapy and HIV in the Genital Tract of Women, G573909246, G5819304971, G6113675784, G702475376, G8523965258, Providence/Boston Center for AIDS Research (CFAR), Center for AIDS Research at Emory University, Women's Reproductive Health Research at the University of Washington
OpenAlex ID: W2165407823