Work

Procedure for determination of free and total cholesterol in micro- or nanogram amounts suitable for studies with cultured cells.

Year: 1978

Type: article

Abstract: Procedures for the determination of free and total cholesterol in lipid extracts or sonicates of 10(4) cultured human skin fibroblasts are described. The method for free cholesterol employs cholesterol oxidase to generate H2O2 and peroxidase to catalyze the reaction of H2O2 with p-hydroxyphenylacetic acid to yield a stable fluorescent product. Cholesterol ester hydrolase is included for determination of total cholesterol. When samples of sonified cell suspensions are used directly, the extraction of lipids is avoided, permitting one person to carry out analyses of 30 or more subcultures in one day.

Source: Journal of Lipid Research

DOI: 10.1016/s0022-2275(20)40693-5

Authors: W Gamble, M Vaughan, H S Kruth, J Avigan

Institutions: National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Cholesterol and Lipid Metabolism

Subfield: Surgery

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W2165482355

FWCI: 13.8

Cites: 9

Cited by: 558

Related to: 10