Work

Conditional Sigma Factor Expression, Using the Inducible Acetamidase Promoter, Reveals that the Mycobacterium tuberculosis sigF Gene Modulates Expression of the 16-Kilodalton Alpha-Crystallin Homologue

Year: 1999

Type: article

Abstract: A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

Source: Journal of Bacteriology

DOI: 10.1128/jb.181.24.7629-7633.1999

Authors: Yukari C. Manabe, Jong Min Chen, Chiew G. Ko, Ping Chen, William R. Bishai

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Awards: G3062614105, G3502070727, G5178890440, G74957556