Work

Story in a sample—the potential (and limitations) of cryo‐electron microscopy applied to molecular machines

Year: 2013

Type: article

Abstract: This article addresses recent developments in cryo-electron microscopy and single-particle reconstruction of macromolecules. With the advent of powerful classification techniques, it is now possible to extract and visualize multiple conformers contained within the same dataset. It is discussed how and to what extent this technique can be used in the study of the dynamics of molecular machines.

Source: Biopolymers

DOI: 10.1002/bip.22274

Author: Joachim Frank

Institutions: Howard Hughes Medical Institute, Columbia University

Language: English

Topic: Advanced Electron Microscopy Techniques and Applications

Subfield: Structural Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institute of General Medical Sciences

Awards: G3030999216, Structural Analysis of Macromolecular Assemblies, G3331595142, Time-resolved Cryo-EM of Short-lived States in Eukaryotic Translation