Cover Picture: Direct Visualization of Lipid Phase Segregation in Single Lipid Bilayers with Coherent Anti‐Stokes Raman Scattering Microscopy (ChemPhysChem 1/2005)
Year: 2005
Type: article
Abstract: The cover picture shows lipid phase segregation in a single giant unilamellar vesicle (GUV) visualized with coherent anti‐Stokes Raman scattering (CARS) microscopy. Phase segregation can be clearly observed, as is evidenced by the CARS image of the deuterated lipid (red) overlaid for clarity with the image of the undeuterated lipid (green). The high sensitivity of CARS microscopy allows visualization of the GUV single lipid bilayer when the Raman shift is tuned into the frequency of the symmetric stretching vibrational mode of CH2, which is abundant in lipid molecules. X. S. Xie et al. show in their Communication on page 77 that CARS microscopy opens up possibilities for intracellular studies of lipid domains.
Source: ChemPhysChem
Authors: Eric O. Potma, X. Sunney Xie
Institution: Harvard University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2165618773