Work

Predicting oligonucleotide affinity to nucleic acid targets

Year: 1999

Type: article

Abstract: A computer program, OligoWalk, is reported that predicts the equilibrium affinity of complementary DNA or RNA oligonucleotides to an RNA target. This program considers the predicted stability of the oligonucleotide-target helix and the competition with predicted secondary structure of both the target and the oligonucleotide. Both unimolecular and bimolecular oligonucleotide self structure are considered with a user-defined concentration. The application of OligoWalk is illustrated with three comparisons to experimental results drawn from the literature.

Source: RNA

DOI: 10.1017/s1355838299991148

Authors: DAVID H. MATHEWS, MARK E. BURKARD, SUSAN M. FREIER, JACQUELINE R. WYATT, DOUGLAS H. TURNER

Institutions: University of Rochester, Ionis Pharmaceuticals (United States)

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Cancer Institute, National Institute of General Medical Sciences

Awards: G1563343907, Folding RNA: from Aids to the Human Genome, University of Rochester Medical Scientist Training Program, Cancer Center Support Grant