Fluorescence Detection of Single‐Nucleotide Polymorphisms with a Single, Self‐Complementary, Triple‐Stem DNA Probe
Year: 2009
Type: article
Abstract: Singled out for its singularity: In a single-step, single-component, fluorescence-based method for the detection of single-nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self-complementary DNA strand that forms a triple-stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F = fluorophore, Q = quencher).
Institution: University of California, Santa Barbara
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2166083795