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The differentiation of hepatic stem cells into pancreatic endocrine tissue: The influence of pancreatic mesoderm

Year: 2008
Type: dissertation
Abstract: The use of adult hepatic stem cells for the treatment of diabetes, based both on the close \nembryological association of the pancreas and liver, and on a putative shared tissue stem \ncell, has been proposed by a number of studies. This study investigated the capacity of \nhepatic oval cells to differentiate into pancreatic endocrine cells in the presence of \npancreatic mesoderm. The GaIN model of hepatic injury was used to induce oval cell \nactivation in Male Sprague-Dawley rats. A viable and significant oval cell population \ncould not however, be isolated and propagated in culture. In order to continue \nexperimentation, a PHeSC-A2 cell line, derived from normal adult porcine liver, was \ncultured with quail pancreatic mesoderm in the GFRM-Ham s F12.ITS culture system. \nCells demonstrating positive immulocalization of the pancreatic markers, insulin and \nglucagon, were identified as PHeSC-A2-derived, by visual assessment of their nuclear \nmorphology. Techniques used to confirm these results and preclude the derivation of the \npancreatic endocrine cells from pancreatic endodermal contamination, proved ineffectual. \nThe tentative results obtained in this study have lead to the following postulations: firstly, \nthe PHeSC-A2 cell line may possess a higher level of potentiality than previously \ndemonstrated; secondly, this potential may be due to the shared embryological origins of \nthe pancreas and liver, and thirdly, permissive signaling from pancreatic mesoderm may \nhave the capacity to induce the differentiation of hepatic oval cells into pancreatic \nendocrine cells. Further research is required to confirm the results obtained in this study \nand to substantiate the aforementioned propositions.
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W2166354375
Cites: 51
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Related to: 10