Antioxidant enzymes in skeletal muscle and their modulation by alcohol and the dehydrogenase inhibitors 4-methylpyrazole and cyanamide
Year: 2010
Type: article
Abstract: High concentrations of alcohol have been shown to adversely affect the metabolism of skeletal muscle but studies into antioxidant status are poorly characterised.We hypothesised that a single exposure to alcohol will impair the antioxidant status of skeletal muscle.We also hypothesised that alcohol-induced defects in muscle will be (i) prevented by 4-methylpyrazole (an inhibitor of alcohol oxidation and thus reduces acetaldehyde formation) and (ii) exacerbated by cyanamide (an inhibitor of acetaldehyde oxidation and thus increases acetaldehyde levels).To test this we used a well-validated rat model of acute alcohol exposure, and then measured total antioxidant capacity of muscle with the Oxygen Radical Absorbance Capacity (ORAC) assay and specific activities of catalase and superoxide dismutase (SOD).We used six groups of rats in the following design.Rats were dosed with a single "pre-treatment" of either saline (control), cyanamide (Cyn; 0.05 mmol/kg BW) or 4-methylpyrazole (4MP; 0.05 mmol/kg BW).After 30 min animals were treated with a single bolus of either saline (0.15 mol/l NaCl, controls) or ethanol (EtOH; 75 mmol/kg BW) then killed 24 h later.The gastrocnemius muscle was dissected and used for analysis.Data were analysed with two-way ANOVA using SPSS v15.
Authors: N. Sumien, J. Crous, J. A. Fegredo, V. R. Preedy
Language: English
Subfield: Pathology and Forensic Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2166618745