Work

Structure of two intramolecular G-quadruplexes formed by natural human telomere sequences in K + solution †

Year: 2007

Type: article

Abstract: Intramolecular G-quadruplexes formed by human telomere sequences are attractive anticancer targets. Recently, four-repeat human telomere sequences have been shown to form two different intramolecular (3 + 1) G-quadruplexes in K(+) solution (Form 1 and Form 2). Here we report on the solution structures of both Form 1 and Form 2 adopted by natural human telomere sequences. Both structures contain the (3 + 1) G-tetrad core with one double-chain-reversal and two edgewise loops, but differ in the successive order of loop arrangements within the G-quadruplex scaffold. Our results provide the structural details at the two ends of the G-tetrad core in the context of natural sequences and information on different loop conformations. This structural information might be important for our understanding of telomere G-quadruplex structures and for anticancer drug design targeted to such scaffolds.

Source: Nucleic Acids Research

DOI: 10.1093/nar/gkm706

Authors: Anh Tuân Phan, Vitaly Kuryavyi, Kim Ngoc Luu, Dinshaw J. Patel

Institutions: Memorial Sloan Kettering Cancer Center, Nanyang Technological University

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,992