Year: 2008
Type: other
Abstract: Although middle cerebral artery (MCA) occlusion in the rat is often used to study focal cerebral ischemia, the model of ischemia aff ects the size and reproducibility of infarction.The purpose of this experiment was to methodically examine diff erent preparations to determine the optimum focal cerebral ischemia model to produce a reproducible severe ischemic injury.Eighty-two Wistar rats underwent 1 hour, 3 hour, or permanent MCA occlusion combined with no, unilateral, or bilateral common carotid artery (CCA) occlusion.Three days after ischemia, the animals were prepared for tetrazolium chloride assessment of infarction size.One-hour MCA occlusion produced a coeffi cient of variation (CV) of 200% with an infarction volume of 20.3 ± 10.5 mm 3 .Adding unilateral or bilateral CCA occlusion resulted in a CV of 134% and 101%, respectively.Three-hour MCA occlusion combined with bilateral CCA occlusion decreased the CV to 58% with a cortical infarction volume of 82.6 ± 12.1 mm 3 , P < 0.05, compared with 1-hour MCA occlusion with or without CCA occlusion.Permanent MCA occlusion combined with 3 hours of bilateral CCA occlusion resulted in a CV of 47% with a cortical infarction volume of 89.6 ± 16.0 mm 3 .These results indicate that 3-hour MCA occlusion combined with bilateral CCA occlusion consistently provides a large infarction volume after temporary focal cerebral ischemia.
Source: Pure Amsterdam UMC
Author: Bert A. Coert
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funder: Strong
OpenAlex ID: W2166736332