Year: 2008
Type: other
Abstract: Endothelial cell-derived microparticles (EMP)Microparticles (MP) are vesicles released from the plasma membrane of many cell types during activation and/or apoptosis.Endothelial cells release EMP, e.g. in response to either TNF-or LPS [34][35][36][37].In vivo, EMP have been reported in peripheral blood from patients suffering from various diseases, such as acute coronary syndrome [38], acuteThe initial aim of this thesis was to establish one or more reliable markers to identify EMP, which preferentially may reflect the status of the (parent) endothelial cells.In Chapter 2, the antigenic phenotype of EMP from activated endothelial cells was characterized and in Chapter 3 we assessed the phospholipid composition of such EMP, and investigated their ability to trigger coagulation in vivo.In Chapter 4, the question was addressed as to which extent EMP originate from adherent or detached endothelial cells, and in Chapter 5 we studied the effects of the cholesterol-lowering drug simvastatin on the relationship between EMP, adherent and detached endothelial cells.Finally, in Chapter 6 we further explored the putative role of EMP formation in endothelial cell survival.Antibodies used were: CD31: platelet endothelial cell adhesion molecule-1 (PECAM-1); CD34: glycoprotein (GP) 105-120; CD41: GPIIb ( IIb ); CD42b: GPIb
Author: M.N. Abid Hussein
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2166854332