Work

A novel DNA-binding motif in MarA: The first structure for an AraC family transcriptional activator

Year: 1998

Type: article

Abstract: A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 A thereby bending the DNA by approximately 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.95.18.10413

Authors: Sangkee Rhee, Robert G. Martin, Judah L. Rosner, David R. Davies

Institutions: National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2166884322

FWCI: 7.983

Cites: 32

Cited by: 300

Related to: 10