Work

Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

Year: 2014

Type: article

Abstract: Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.

Source: Chemical Communications

DOI: 10.1039/c4cc07778d

Authors: Frank J. Piscotta, Jeffery M. Tharp, Wenshe R. Liu, A. James Link

Institutions: Princeton University, Texas A&M University

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Welch Foundation, National Institute of General Medical Sciences, Division of Chemical, Bioengineering, Environmental, and Transport Systems, Division of Chemistry

Awards: G4381921026, G556965016, G6553094674, G7455009664