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Mapping and Serodiagnostic Application of a Dominant Epitope within the Human Herpesvirus 8 ORF 65-Encoded Protein

Year: 1998

Type: article

Abstract: A dominant epitope within the human herpesvirus 8 (HHV8) ORF 65-encoded protein was mapped to an 8-amino-acid (aa) sequence (RKPPSGKK [aa 162 to 169]) by an amino acid replacement method. Using a 14-aa peptide (P4) encompassing this epitope as the antigen, we developed an enzyme immunoassay for HHV8 antibodies. The presence of P4 antibodies in a panel of 61 human serum specimens was highly correlated with biopsy-confirmed Kaposi's sarcoma. The homologous Epstein-Barr virus peptide derived from BFBR3-encoded protein did not interfere with the assay, suggesting that P4 is specific for HHV8.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.36.6.1574-1577.1998

Authors: Chou-Pong Pau, Lee L. Lam, Thomas J. Spira, Jodi B. Black, John A. Stewart, Philip E. Pellett, Richard A. Respess

Institutions: Centers for Disease Control and Prevention, National Center for Infectious Diseases

Language: English

Topic: Viral-associated cancers and disorders

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2167163352

FWCI: 2.484

Cites: 23

Cited by: 70

Related to: 10