Year: 2012
Type: article
Abstract: Interchromosomal chimeric RNA molecules are often transcription products from genomic rearrangement in cancerous cells. Here we report the computational detection of an interchromosomal RNA fusion between ZC3HAV1L and CHMP1A from RNA-seq data of normal human mammary epithelial cells, and experimental confirmation of the chimeric transcript in multiple human cells and tissues. Our experimental characterization also detected three variants of the ZC3HAV1L-CHMP1A chimeric RNA, suggesting that these genes are involved in complex splicing. The fusion sequence at the novel exon-exon boundary, and the absence of corresponding DNA rearrangement suggest that this chimeric RNA is likely produced by trans-splicing in human cells.
Source: Biology Direct
Institution: Princeton University
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,890
Awards: RNA-GUIDED DNA REARRANGEMENTS IN BREAST CANCER, G1939566967, G2266079415, G232496113, The Role of miRNAs in Epithelial-Mesenchymal Transition and Metastasis, G4119534169, G5594648797, G5850682332, G6084365361, Understanding Complex Gene Editing Systems in Protists, Metadherin in Metastasis and Chemoresistance of Breast Cancer, Epigenetic Mechanisms for the Inheritance of Acquired Mutations, Collaborative Research: RNA-guided DNA recombination through assembly graphs, G8777012361, G9000263413
OpenAlex ID: W2167207114