Application of a Monoclonal Antibody to a Comparative Study of α-Lactalbumins from Various Species
Year: 1989
Type: article
Abstract: A monoclonal antibody to bovine alpha-lactalbumin was prepared and purified. The binding ability of alpha-lactalbumin from different species (cow, goat, giraffe, horse, pig, human, monkey, and guinea pig) was examined by a competitive radioimmunoassay. The order in strength of the binding affinity was cow goat, giraffe, horse, cynomolgus monkey and human, pig, and guinea pig. The order of evolutional divergence calculated from the amino acid composition was cow, goat, giraffe, horse, pig, guinea pig and human, and monkey. The orders in both cases were similar. Hence, it is suggested that immunological divergence as deduced by a monoclonal antibody is likely to be close to the evolutional divergence of alpha-lactalbumin.
Source: Journal of Dairy Science
Authors: Shuichi Kaminogawa, Michiko Shimoda, Jun-Ichi Kurisaki, Kunio Yamauchi
Institution: The University of Tokyo
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2167604723