Year: 2003
Type: article
Abstract: The steps involved in lymphocyte homing to the white pulp cords of the spleen are poorly understood. We demonstrate here that the integrins lymphocyte function associated (LFA)-1 and alpha 4 beta 1 make essential and mostly overlapping contributions necessary for B cell migration into white pulp cords. T cell entry to the white pulp is also reduced by blockade of LFA-1 and alpha 4 beta 1. The LFA-1 ligand, intercellular adhesion molecule 1 is critical for lymphocyte entry and both hematopoietic cells and radiation-resistant cells contribute to this requirement. Vascular cell adhesion molecule 1 contributes to the alpha 4 beta 1 ligand requirement and a second ligand, possibly fibronectin, also plays a role. By contrast with the entry requirements, antigen-induced movement of B cells from follicles to the outer T zone is not prevented by integrin blocking antibodies. Comparison of the distribution of integrin-blocked B cells and B cells treated with the G alpha i inhibitor, pertussis toxin, early after transfer reveals in both cases reduced accumulation in the inner marginal zone. These observations suggest that chemokine receptor signaling and the integrins LFA-1 and alpha 4 beta 1 function together to promote lymphocyte transit from the marginal zone into white pulp cords.
DOI: 10.1084/jem.20021569
Authors: Charles G. Lo, Theresa T. Lu, Jason G. Cyster
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Awards: IN VITRO AND ANIMAL MODELS FOR EMERGING DISEASES AND BIODEFENSE, G3156151498, G4965763455, Chemokines and Lymphoid Tissue Organization and Function, G9000221878, G9000344376, B lymphocyte Migration and Homeostasis, Pediatric Scientist Development Program, Chemokines and Lymphoid Tissue Organization and Function
OpenAlex ID: W2167808722