Year: 2012
Type: article
Abstract: Downstream regulatory element antagonist modulator (DREAM) is a Ca(2+)-binding protein that binds DNA and represses transcription in a Ca(2+)-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca(2+) homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca(2+)-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function.
Institutions: Barcelona Biomedical Research Park, Centre for Biomedical Network Research on Rare Diseases, Centre for Genomic Regulation, University of Padua, Consejo Superior de Investigaciones Científicas, Centro Nacional de Biotecnología, Veneto Institute of Molecular Medicine, Biomedical Research Networking Center on Neurodegenerative Diseases
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
Award: G2177838879
OpenAlex ID: W2167825053